circ_0029463 promotes osteoclast differentiation by mediating miR-134-5p/Rab27a axis

circ_0029463 promotes osteoclast differentiation by mediating miR-134-5p/Rab27a axis

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Abstract Objective Osteoporosis is the imbalance in bone homeostasis between osteoblasts and osteoclasts.In this study, we investigated the effects of the circ_0029463/miR-134-5p/Rab27a axis on RANKL-induced osteoclast differentiation.Methods RT-qPCR and western blotting were used to detect the expression of circ_0029463, miR-134-5p, and Rab27a in tissues from patients with osteoporosis and in RANKL-induced osteoclasts.

Osteoclast differentiation was verified by TRAP staining.Osteoclast biomarkers, including NFATc1, TRAP, and CTSK, were measured.The target and regulatory relationships between circ_0029463, miR-134-5p, and the Rab27a Jandy LED Laminar Parts axis were verified using RIP, dual-luciferase reporter gene, and RNA pull-down assays.

Results Elevated expression of circ_0029463 and Rab27a and Massage Therapy decreased miR-134-5p expression were observed in the tissues of patients with osteoporosis, and a similar expression pattern was observed in RANKL-induced osteoclasts.Suppression of circ_0029463 expression or miR-134-5p overexpression curbed RANKL-induced osteoclast differentiation, whereas such an effect was abolished by Rab27 overexpression.circ_0029463 sponges miR-134-5p to induce Rab27a expression.

Conclusion circ_0029463 sponges miR-134-5p to abolish its suppressive effect of miR-134-5p on Rab27a expression, thereby promoting osteoclast differentiation.